Coding

Part:BBa_K1365001:Design

Designed by: Sandra Mous   Group: iGEM14_Groningen   (2014-09-24)


NisB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2007


Design Notes

Used primers:

forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA-3'

reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT-3'

to PCR NisB from the genome and add the BioBrick prefix and suffix.


Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

References